Wheat (Triticum aestivum L.) is the most precious staple food and is highly regarded in the world as king of cereals. The present experiment was conducted to determine the combining ability analysis involving six high yielding lines of wheat ( Triticum aestivm L.) viz., 9481, 9486, 9490, 9862, 9863 and 9864 and three testers Pasban-90, Galaxy -13 and Millat-11 which were crossed in line × tester mating design in the experimental area of the Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad. The parental genotypes and their F 1 hybrids were examined in randomized complete block design (RCBD), with three replications under normal conditions in the season 2015 -16. The data were collected for stomata and quantitative indices viz., plant height, flag leaf area, stomata size, stomata frequency, 1000-grain weight and grain yield per plant. The data were analyzed statistically and combining ability studies were done using line × tester analysis, highly significant differences were shown among the lines and testers for most of the traits. For flag leaf area, stomata size, stomata frequency and grain yield per plant genotype 9481 proved to be good general combiner among the female parent. Among testers (males) Galaxy -13 exhibited positive general combining ability for the studied traits. Among the crosses, the hybrid 9481 × Galaxy-13 was the best specific combiner for most of the yield contributing traits. The superior genotypes and crosses can be combined to develop new promising and improved wheat varieties for obtaining high yield.
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