Burn is one of the most common and devastating forms of trauma which is caused by severe thermal injury to skin and soft tissue. It has been estimated that abo ut 75% of the mortality associated with burn injuries is related to sepsis especially in developing countries. Multi Drug Resistant (MDR) Pseudomonas aeruginosa is the most commonly isolated pathogen from burn wounds. The present study was designed to isolate and characterize the MDR isolates of Pseudomonas aeruginosa followed by evaluating the antibacterial effect of green tea extracts against the isolated pathogens. A total of 65 samples from burn wound patients were collected for isol ation of Pseudomonas aeruginosa using Pseudomonas agar. The samples were confirmed using biochemical tests. The antibiotic sensitivity testing and antimicrobial potential of green tea extracts ( aqueous and n -hexane) against isolated P. aeruginosa was determined by using Kirby-Bauer disc diffusion method . Pseudomonas aeruginosa was isolated from 23 (35.4%) samples and among these 10 (44%) isolates were multi drug resistant. The Pseudomonas aeruginosa isolates were found 100% resistant to ceftriazone (CRO), tobramycin (TOB) and piperacillin -tazobactam (TZP) while majority of the isolates were intermediately resistant to other commonly used antibiotics. Ciprofloxacin was found to be the most effective drug which was sensitive to 50% of isolates. The n-hexane extract of green tea showed good antibacterial activity having mean zone of inhibition (ZOI) 21.5±0.84mm and 21.8±0.63mm at 100mg/ml and 200mg/ml concentrations as compared to aqueous extracts. This study showed the high frequency of multidrug resistant Pseudomonas aeruginosa in burn wounds and revealed that n - hexane extract of green tea is a good substitute to antibiotics in the management of MDR P. aeruginosa infections in future.
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