Anti-inflammatory Steroidal Saponins from Panicum turgidum

Abstract

The bio-guided fractionation and phytochemical investigation of Panicum turgidum extract resulted in the isolation of three steroidal saponins , identified as yamogenin-3β-O-α-L-rhamnopyranosyl-(12)-O-[α-L- rhamnopyranosyl-(14)]-β-D-glucopyranoside ( 1), yamogenin-3β-O-α-L-rhamnopyranosyl-(12)-O-[α-L- rhamnopyranosyl-(14)-rhamnopyranosyl-(14)]-β-D-glucopyranoside ( 2), and diosgenin-17α-hydroxy- 3β-O-α-L-rhamnopyranosyl-(12)-O-[α-L-rhamnopyranosyl-(14)]-β-D-glucopyranoside (3). Their structures were established on the basis of spectroscopic methods including 1D and 2D NMR and HRESIMS. The isolated compounds were tested for anti -inflammatory activities by iNOS inhibition assay. Compound 2 showed potent activity with IC 50 2.1 μM, followed by compound s 3 and 1 with IC 50 8.6 and 16 μM, respectively.

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