To assess the effect of nitrogen rate on growth, yield and nitrogen use efficiency of some Bt cotton cultivars, a field trial was conducted during 2014 at Students’ Research Farm, Department of Agronomy, University of Agriculture Faisalabad Pakistan. Experiment was laid out in randomized complete block design (RCBD) with factorial arrangement using three cotton cultivars (FH -114, FH -142 and MNH -886) under various nitrogen rates (150, 200 and 250 kg N ha -1). All the data were recorded and analyzed statistically using standard procedures. Different cotton cultivars at varying nitrogen doses significantly affected the growth, deve lopment and seed cotton yield. Crop took maximum time for first squaring (45.33 days), flower initiation (62 days and boll splition (188.56 days) with 250 kg N ha -1. Leaf area index (3.63) and number of sympodial branches per plant were also highest at nitrogen level of 250 kg ha -1 while total dry matter (12847 kg ha -1), number of monopodial branches per plant (3.77), earliness index (43.74%) and seed cotton yield were recorded higher at 200 kg N ha -1. In case of cultivars, FH -142 produced more seed cott on yield (3369 kg ha -1), total dry matter (TDM) (13461 kg ha -1) and leaf area index (LAI) (3.69) but maximum number of days to first boll splition (118 days) was noted in MNH -886. Time period for flower initiation, earliness index, monopodials and sympodials remained non-significant among cultivars.
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