Among the most important and widely-cultivated crops in the world, wheat ranks third after maize and rice. The global consumption of wheat increased during the past decades and it is expected that demand for major grains, such as maize, rice, and wheat will increase 48% by 2025. The constraints limiting wheat production are associated with several biotic and abiotic stresses. Karnal Bunt (KB) disease has got a significant importance and plays a major role in deteriorating the quality of wheat grain. Coefficient of similarity and genetic distances among different sets of materials was calculated by using Unweighted Pair Gro up of arithmetic means (UPGMA) function. For the assessment of variations for rust resistance among cultivar, 52 genotypes were used for RAPD primers. Out of provided primers OpA -15(TTCCGAACCC), OpB - 5(TGCGCCCTTC) and OpG -2(GGCACTGAGG) proved result oriente d. These primers have detected total 18 loci and out of these, 11 were polymorphic with a polymorphism of 61.11%. The cluster analysis of this whole dendogram reveals that genotypes pop -49 (SABUF/7/ALTAR84/ AE. SQUARROSA(224)//YACO/6/CROC_1/AE. SQUARR -OSA(205)/5/BR12*3/4/...?) and pop -52 (369) BCN/3/68112/WARD//AE. SQUARROSA) are showing maximum genetic diversity. These two genotypes are showing a maximum genetic distance of 9.12% as compared with all other genotypes included in analysis.
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