F1 seed mutagenesis: A novel technique of creating genetic variability in lentil

Abstract

Plant genetic resources are the guarantee of the world f ood security. Genetic re sources possess genetic variability contained in traditional varieties, cultivars, wild crop relatives and other wild species. Hybridization is the conventional method used to create genetic variation . Induced mutations are also a good source of creating genetic variability . In F 1 seed mutagenesis , F1M0 seeds are mutated with different doses of mutagens (EMS or Gamma rays) and are planted in the field to get F 2M1 generation and then F 2M1 seeds are planted in the field to get F 2M3 generation. Segregating generation (F2M3) creates a large amount of genetic variation desired by the plant breeders to develop high -yielding and disease resistant varieties. We have recommended that combination of both these approaches i.e. hybridization and induced mutations ( F1 seed mutagenesis) will create a large amount of genetic variation in lentil. Breeding scheme for F1 seed mutagenesis was also given. Breeders should use this novel strategy for creating genetic variation in lentil as well as in other crops.

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